| Citation |
Ryder, E., Blows, F., Ashburner, M., Bautista-Llacer, R., Coulson, D., Drummond, J., Webster, J., Gubb, D., Gunton, N., Johnson, G., O'Kane, C.J., Huen, D., Sharma, P., Asztalos, Z., Baisch, H., Schulze, J., Kube, M., Kittlaus, K., Reuter, G., Maroy, P., Szidonya, J., Rasmuson-Lestander, A., Ekstrom, K., Dickson, B., Hugentobler, C., Stocker, H., Hafen, E., Lepesant, J.A., Pflugfelder, G., Heisenberg, M., Mechler, B., Serras, F., Corominas, M., Schneuwly, S., Preat, T., Roote, J., Russell, S. (2004). The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. Genetics 167(2): 797--813. |
| PubMed Abstract |
We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations
in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously
mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the
utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution,
to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens
where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for
designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate
>12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable
by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that
is precisely mapped to the genome sequence.
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