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Reference
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| Citation |
Sawicki, R., Singh, S.P., Mondal, A.K., Benes, H., Zimniak, P. (2003). Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione
S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class. Biochem. J. 370(2): 661--669. |
| FlyBase ID |
FBrf0158728 |
| Type of publication |
Research paper |
| Offprint Available |
No |
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External Crossreferences
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| PubMed ID |
12443531 |
| PubMed Abstract |
From the fruitfly, Drosophila melanogaster, ten members of the cluster of Delta-class glutathione S-transferases (GSTs; formerly
denoted as Class I GSTs) and one member of the Epsilon-class cluster (formerly GST-3) have been cloned, expressed in Escherichia
coli, and their catalytic properties have been determined. In addition, nine more members of the Epsilon cluster have been
identified through bioinformatic analysis but not further characterized. Of the 11 expressed enzymes, seven accepted the lipid
peroxidation product 4-hydroxynonenal as substrate, and nine were active in glutathione conjugation of 1-chloro-2,4-dinitrobenzene.
Since the enzymically active proteins included the gene products of DmGSTD3 and DmGSTD7 which were previously deemed to be
pseudogenes, we investigated them further and determined that both genes are transcribed in Drosophila. Thus our present results
indicate that DmGSTD3 and DmGSTD7 are probably functional genes. The existence and multiplicity of insect GSTs capable of
conjugating 4-hydroxynonenal, in some cases with catalytic efficiencies approaching those of mammalian GSTs highly specialized
for this function, indicates that metabolism of products of lipid peroxidation is a highly conserved biochemical pathway with
probable detoxification as well as regulatory functions.
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| Biosis |
2003.164533 |
| Zoological record |
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Associated Information
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| Text of personal communication |
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
Biochem. J. |
| Title |
Biochemical Journal |
| Authors |
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| Volume range |
1- |
| Year range |
1906- |
| Page range |
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| Publisher |
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| Place of publication |
London |
| Language of publication |
English |
| ISBN/ISSN |
0264-6021 |
| CODEN |
BIJOAK |
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Data from Reference
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| Genes (21)
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| Transcripts (2)
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