Reference Report

Reference
Citation	Gubb, D., Green, C., Huen, D., Coulson, D., Johnson, G., Tree, D., Collier, S., Roote, J. (1999). The balance between isoforms of the prickle LIM domain protein is critical for planar polarity in Drosophila imaginal discs. Genes Dev. 13(17): 2315--2327.
FlyBase ID	FBrf0111378
Type of publication	Research paper
Offprint Available	No
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PubMed ID	10485852
PubMed Abstract	The tissue polarity mutants in Drosophila include a set of conserved gene products that appear to be involved in the control of cytoskeletal architecture. Here we show that the tissue polarity gene prickle (pk) encodes a protein with a triple LIM domain and a novel domain that is present in human, murine, and Caenorhabditis elegans homologs which we designate PET. Three transcripts have been identified, pk, pkM, and sple, encoding 93-, 100-, and 129-kD conceptual proteins, respectively. The three transcripts span 70 kb and share 6 exons that contain the conserved domains. The pk and sple transcripts are expressed with similar tissue-specific patterns but have qualitatively different activities. The phenotypes of pk mutants, and transgenic flies in which the different isoforms are overexpressed show that the balance between Pk and Sple is critical for the specification of planar polarity. In addition, these phenotypes suggest a tessellation model in which the alignment of wing hairs is dependent on cell shape and need not reflect fine-grained positional information. Lack of both pk and sple transcripts gives a phenotype affecting the whole body surface that is similar to those of dishevelled and frizzled (fz) suggesting a functional relationship between pk and fz signaling.
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Language of publication	English
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Abbreviation	Genes Dev.
Title	Genes & Development
Authors
Volume range	1-
Year range	1987-
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Place of publication	Cold Spring Harbor, NY
Language of publication	English
ISBN/ISSN	0890-9369
CODEN	GEDEEP
Data from Reference
Aberrations (33)
	Df(2R)CA58 Df(2R)Drl^rv30 Df(2R)cn76k3 Df(2R)nap2 Df(2R)pk-N5 Df(2R)pk-sple-50 Df(2R)pk-sple-51 Df(2R)pk78k Df(2R)pk³⁰ Df(2R)sple-D1 Df(2R)sple-D2 Df(2R)sple-J1 Df(2R)sple-J2 In(1)m^38c In(2LR)O In(2LR)TE35B-4 In(2LR)pk-20 In(2LR)pk-21 In(2LR)pk-sple⁴⁷ In(2LR)pk⁴⁴ In(2R)nec-5 In(2R)nec-e3 In(2R)pk-19 In(2R)pk-sple⁴⁰ T(2;3)cn88e43 T(2;3)pk-sple³¹ T(2;3)pk-sple³⁷ T(2;3)pk²⁹ T(Y;2)pk-sple⁴⁵ Tp(2;2)pk-sple²² Tp(2;2)pk-sple²⁴ Tp(2;2)pk-sple²⁶ Tp(2;2)pk-sple⁴⁹
Alleles (66)
	Scer\GAL4^C-765 Scer\GAL4^da.G32 Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^pnr-MD237 Spn43Ab³⁰ dsh^unspecified fz^unspecified m^38c pk¹⁵ pk¹⁶ pk¹⁷ pk¹⁹ pk¹ pk²⁰ pk²¹ pk²⁹ pk² pk³⁰ pk³ pk⁴⁴ pk⁵ pk^EP2557 pk^SZ4 pk^Scer\UAS.cGa pk^cn76k3 pk^cn88e43 pk^nap2 pk^pk-sple-10 pk^pk-sple-11 pk^pk-sple-12 pk^pk-sple-13 pk^pk-sple-14 pk^pk-sple-18 pk^pk-sple-22 pk^pk-sple-23 pk^pk-sple-25 pk^pk-sple-26 pk^pk-sple-28 pk^pk-sple-32 pk^pk-sple-33 pk^pk-sple-34 pk^pk-sple-35 pk^pk-sple-37 pk^pk-sple-38 pk^pk-sple-39 pk^pk-sple-40 pk^pk-sple-41 pk^pk-sple-43 pk^pk-sple-46 pk^pk-sple-47 pk^pk-sple-48 pk^pk-sple-49 pk^pk-sple-6 pk^pk-sple-7 pk^pk-sple-8 pk^pk-sple-9 pk^sple-1 pk^sple-27 pk^sple-36 pk^sple-3 pk^sple-42 pk^sple-4 pk^sple-5 pk^{sple.Scer\UAS} w^+mC
Balancers (6)
	CyO CyO-G CyO-pk^pk-sple-46 CyO-pk^pk-sple-47 CyO-pk^pk-sple-48 CyO-pk^pk-sple-49
Constructs (4)
	P{GAL4-da.G32} P{GAL4-dpp.blk1} P{UAS-pk.G} P{UAS-sple⁺}
Genes (12)
	Ady43A Scer\GAL4 Spn43Aa Spn43Ab dsh esn fz m nec pk pwn w
Insertions (7)
	P{EP}pk^EP2557 P{GawB}C-765 P{GawB}pnr^MD237 P{en2.4-GAL4}e16E P{}pk¹⁶ P{}pk¹⁷ P{}pk^pk-sple-33