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Reference
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| Citation |
Toung, Y.P., Hsieh, T.S., Tu, C.P. (1993). The glutathione S-transferase D genes. A divergently organized, intronless gene family in Drosophila melanogaster. J. Biol. Chem. 268(13): 9737--9746. |
| FlyBase ID |
FBrf0058802 |
| Type of publication |
Research paper |
| Offprint Available |
Yes |
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External Crossreferences
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| PubMed ID |
7683659 |
| PubMed Abstract |
We have characterized a cluster of glutathione S-transferase genes located at 87B on the Drosophila polytene chromosome near
the heat shock genes, hsp70. These genes, designated gst Ds in the glutathione S-transferase gene superfamily, are closely
linked within a approximately 60-kilobase DNA segment. The gene family has a minimum of eight intronless genes organized in
divergent orientations. Two of the genes are probably GST pseudogenes in that their open reading frames are shorter than functional
GSTs, and no RNAs from them have been detected thus far. The amino acid sequence identity among the functional genes ranges
from 53 to 75% in pairwise comparisons. The intergenic regions are much more AT rich (63-73%) than the coding regions (41-52%),
consistent with being promoter/regulatory sequences in Drosophila melanogaster. The mRNA size for each gene suggests that
these genes are probably expressed individually from separate promoters. This is the first documentation of definitive physical
linkage of a functional glutathione S-transferase multigene family. The genes are divergently organized, and a gradation of
sequence similarity exists among the encoded GST isozymes. The patterns of sequence similarity in pairwise comparisons of
the family members suggest that gene conversion may have played a role in the evolution of this GST multigene family. We propose
that the Drosophila gst D genes provide a unique system for studying GST gene regulation, in vivo physiological functions,
and evolution of substrate specificities with a global perspective. The gst D genes in other organisms should be intronless
and can be isolated directly from genomic DNAs for functional analyses at the gene and protein levels.
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| Biosis |
96027896 |
| Zoological record |
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Associated Information
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| Text of personal communication |
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Also Published As
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Other Reference Information
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| Secondary IDs |
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| Language of publication |
English |
| Additional language(s) of abstract |
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| ISBN |
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| Place of publication |
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Published In
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| Abbreviation |
J. Biol. Chem. |
| Title |
Journal of Biological Chemistry |
| Authors |
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| Volume range |
1- |
| Year range |
1905- |
| Page range |
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| Publisher |
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| Place of publication |
Baltimore, MD |
| Language of publication |
English |
| ISBN/ISSN |
0021-9258 |
| CODEN |
JBCHA3 |
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Data from Reference
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| Genes (9)
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