Gene Dmel\GstE2

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General Information

Symbol

Dmel\GstE2

Species

D. melanogaster

Name

Glutathione S transferase E2

Annotation symbol

CG17523

Feature type

protein_coding_gene

FlyBase ID

FBgn0063498

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

55C6-55C7

Sequence location

2R:14,286,993..14,287,884 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Glutathione S transferase E2 is referred to in FlyBase by the symbol GstE2 (CG17523, FBgn0063498). It has the cytological map location 55C6-55C7. Its sequence location is 2R:14286993..14287884. Its molecular function is described as glutathione transferase activity. The biological processes in which it is involved are not known. One allele is reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55C6-55C7

Limits computationally determined from genome sequence between P{PZ}sbb⁰⁴⁵²⁵&P{EP}EP1081^EP1081 and P{lacW}l(2)08770^k04808

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: GstE10- GstE1+ GstE2+ GstE3+ GstE4+ GstE5+ GstE6+ GstE7+ GstE8+ GstE9+

(Sawicki et al., 2003)

Gene Model & Products

Please see the GBrowse view of Dmel\GstE2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

GstE2-RA

FBtr0086670

NM_137480

892

221

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

GstE2-PA

FBpp0085851

25.2

221

5.34

NP_611324

AAF57700

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q7JYZ9

Maps to

Does NOT map to

Identified with

RE69679

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0063498

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0063498

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of GstE2	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of GstE2	Class	Mutagen	Stocks	Known lesion
GstE2^GD9404		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)PC4

Useful duplication

Dp(2;Y)53D;57C

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9404}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

glutathione transferase activity

inferred from sequence or structural similarity with EMBL:U66342

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

GstE2 allele

Gene

References

External Data

Linkouts

Orthologs

Genome-wide drosophilid orthologs

Dana\GF12162

Dere\GG21882

Dgri\GH15974

Dper\GL17769

Dpse\GA14540

Dsec\GM21875

Dsim\GD11372

Dvir\GJ12879

Dwil\GK22982

Dyak\GstE2

Curated drosophilid orthologs

Dpse\GA14540

Dyak\GstE2

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

VDRC

v32945

w¹¹¹⁸; P{GD9404}v32945

Genomic Clones ( 1 )

BACR48A20

cDNA Clones ( 2 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RE69679

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE63657

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0063498

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_137480

RefSeq (Proteins)

•

NP_611324

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q7JYZ9

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0063498

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0063498

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0063498

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0063498

Synonyms & Secondary IDs ( 4 )

Reported As

Symbol Synonym

CG17523

(Ranson et al., 2001, Asha et al., 2003, )

DmGSTE2

(Sawicki et al., 2003, Zimniak and Benes, 2002.7.15)

GstE2

Name Synonym

Glutathione S transferase E2

(Zimniak and Benes, 2002.7.15)

Secondary FlyBase IDs

FBgn0034336

References ( 9 )

Research paper

Asha et al., 2003, Genetics 163(1): 203--215: Analysis of ras-induced overproliferation in Drosophila hemocytes. [FBrf0155813]
Sawicki et al., 2003, Biochem. J. 370(2): 661--669: Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class. [FBrf0158728]
Ranson et al., 2001, Biochem. J. 359(2): 295--304: Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae. [FBrf0151633]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Zimniak and Benes, 2002.7.15, Insect GSTs.: Insect GSTs. [FBrf0150782]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]

Curated genome annotation

Gong, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126669]