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Gene Dmel\ATPCL

General Information
Symbol	Dmel\ATPCL	Species	D. melanogaster
Name	ATP citrate lyase	Annotation symbol	CG8322
Feature type	protein_coding_gene	FlyBase ID	FBgn0020236
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	52D9-52D11	Sequence location	2R:11,879,931..11,888,765 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene ATP citrate lyase is referred to in FlyBase by the symbol ATPCL (CG8322, FBgn0020236). It has the cytological map location 52D9-52D11. Its sequence location is 2R:11879931..11888765. Its molecular function is described as: ATP citrate synthase activity; succinate-CoA ligase (ADP-forming) activity; oxygen binding; ATP binding. It is involved in the biological processes: acetyl-CoA biosynthetic process; citrate metabolic process; cellular carbohydrate metabolic process. 9 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 52D9-52D11   Limits computationally determined from genome sequence between P{lacW}l(2)k02205k02205&P{PZ}sli05248 and P{lacW}ATPCLk09217
Experimentally Determined Cytological Location
Cytogenetic map Notes References 52D9-52D10 (determined by in situ hybridisation) 52D11--12 (determined by in situ hybridisation)   (Spradling et al., 1999) 52D9-52D10 52D11--12   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\ATPCL for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ATPCL-RA FBtr0087265  NM_079031   5143   1086 ATPCL-RB FBtr0087266  NM_166131   5260   1086 ATPCL-RC FBtr0290132     5191   1102
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein ATPCL-PA   FBpp0086403   118.6   1086   7.23     NP_523755   AAF58082 ATPCL-PB   FBpp0086404   118.6   1086   7.23     NP_725514   AAM70940 ATPCL-PC   FBpp0288571   120.2   1102   7.45
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0020236
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Leghaemoglobin (IPR001032) CoA-binding (IPR003781) Succinyl-CoA synthetase, beta subunit (IPR005809) Succinyl-CoA ligase, alpha subunit (IPR005810) ATP-citrate lyase/succinyl-CoA ligase (IPR005811) ATP-grasp fold, succinyl-CoA synthetase-type (IPR013650) ATP-grasp fold, subdomain 2 (IPR013816) ATP-citrate synthase (IPR014608) Succinyl-CoA synthetase-like (IPR016102) Citrate synthase-like, core (IPR016141) Citrate synthase-like, small alpha subdomain (IPR016143) ATP-citrate lyase/succinyl-CoA ligase, active site (IPR017440) NAD(P)-binding (IPR016040)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695049   AA697678   AA735448   AC004426   AC004516   AE003808 AAF58082   AE003808 AAM70940   AF132166 AAD34754   AI403661   AI405870   AI455129   AI541859   AQ025890   AQ034162   AQ073497   AX154830   BF502123   BI162207   BI162208   BI565292   BI576638   BI583256   BI604329   BI605742   BI610618   BI618965   BI621310   BI633319   BP552353   BP552354   BT010203 AAQ23521   BT015200   CK657000   CK660003   CO177048   CO187135   CO188767   CO268664   CO270419   CO276762   CO276788   CO282943   CO287154   CO291817   CO291884   CO302072   CO310027   CO316474   CO316927   CO320933   CO326024   CO329985   CO341223   CZ468218   CZ479244   CZ479245   CZ482011   EC057517   EC062698   EC063545   EC064445   EC067419   EC069266   EC069693   EC073769   EC074617   EC075835   EC078001   EC082250   EC082320   EC089386   EC199798   EC201787   EC210990   EC215359   EC217839   EC224023   EC230143   EC232170   EC236500   EC242789   EC242851   EC243540   EC245878   EC250627   EC252154   RE01584   U87317 AAB47486
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q6NR74 Q7JNC6 Q7KN85 A1ZA78
Maps to	DS08018 (BDGP Project Members, 1994-1999)
Does NOT map to
Identified with	LD21334 (Spradling et al., 1999, BDGP Project Members, 2000-) SD07805 (BDGP Project Members, 2000-) Dm0527 (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 12322
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AI405870
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020236 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0020236 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0020236
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable ATPCLk09217 lethal | recessive ATPCL01466 semi-lethal ATPCL01466 Phenotype manifest in Allele
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of ATPCL Class Mutagen Stocks Known lesion ATPCL01466 P-element activity 1 Yes ATPCLDG23402 P-element activity 1 -- ATPCLEP2329 P-element activity 1 -- ATPCLMB01163 1 -- ATPCLc02804 piggyBac transposase 1 -- ATPCLe01432 piggyBac transposase 1 -- ATPCLe04264 piggyBac transposase 0 -- ATPCLk09217 P-element activity 2 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ATPCL Class Mutagen Stocks Known lesion ATPCLGD3552 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes
Aneuploid Aberrations
Useful duplication	Dp(2;2)SMG45
Disrupted in	Df(2R)Exel7138 (Ryder, 2004.4.26) Df(2R)Jp1 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)Jp4 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)Jp6 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD17534} NA P{GD3552} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}ATPCLEP2329 NA P{GSV1}EP-M21 NA miscellaneous insertions PBac{PB}ATPCLc02804 NA PBac{RB}ATPCLe01432 NA PBac{RB}ATPCLe04264 NA P{GT1}BG00805 NA P{PTT-GB}CB04427 NA P{SUPor-P}KG00786 NA P{wHy}ATPCLDG23402 NA insertion of enhancer trap binary system Mi{ET1}CG8320MB01163 No insertion of enhancer trap P{PZ}ATPCL01466 Yes P{lacW}ATPCLk09217 No P{lacW}l(2)SH0527SH0527 No
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 9 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR013816 (FlyBase Curators et al., 2004-)
ATP citrate synthase activity inferred from sequence or structural similarity (Spradling et al., 1999) non-traceable author statement (Ryerse, 1997.1.27) inferred from sequence or structural similarity with UniProt:P16638 (FlyBase, 1992-)
oxygen binding inferred from electronic annotation with InterPro:IPR001032 (FlyBase Curators et al., 2004-)
succinate-CoA ligase (ADP-forming) activity inferred from electronic annotation with InterPro:IPR017440 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
acetyl-CoA biosynthetic process inferred from sequence or structural similarity with UniProt:P16638 (FlyBase, 1992-)
cellular carbohydrate metabolic process inferred from electronic annotation with InterPro:IPR014608, InterPro:IPR016141, InterPro:IPR016143 (FlyBase Curators et al., 2004-)
citrate metabolic process inferred from sequence or structural similarity with UniProt:P16638 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
cytoplasm inferred from sequence or structural similarity with UniProt:P16638 (FlyBase, 1992-)
lipid particle inferred from direct assay (Beller et al., 2006) inferred from direct assay (Beller et al., 2006)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ATPCL allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 62486
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13329 Dere\GG22311 Dgri\GH19829 Dmoj\GI20417 Dper\GL20648 Dpse\GA20986 Dsec\GM20101 Dsim\GD25577 Dvir\GJ20089 Dwil\GK10643 Dyak\GE14108
Curated drosophilid orthologs	Dpse\GA20471   Dpse\GA20986
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000012364 Anopheles gambiae (Ensembl) ENSBTAP00000022256 Bos taurus (Ensembl) ENSCAFP00000023291 Canis familiaris (Ensembl) ENSCINP00000018161 Ciona intestinalis (Ensembl) NEWSINFRUP00000135555 Takifugu rubripes (Ensembl) ENSGALP00000005492 Gallus gallus (Ensembl) ENSP00000253792 Homo sapiens (Ensembl) ENSMMUP00000024909 Macaca mulatta (Ensembl) ENSMODP00000018423 Monodelphis domestica (Ensembl) ENSPTRP00000044752 Pan troglodytes (Ensembl) ENSRNOP00000023447 Rattus norvegicus (Ensembl) GSTENP00012625001 Tetraodon nigroviridis (Ensembl) ENSXETP00000004895 Xenopus tropicalis (Ensembl) Q93VT8 Oryza sativa (Gramene) AT3G06650.1 Arabidopsis thaliana (Arabidopsis) AT5G49460.1 Arabidopsis thaliana (Arabidopsis) WBGene00031017 Caenorhabditis briggsae (Wormbase) WBGene00007150 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040718-436 Danio rerio (Zfin) ZDB-GENE-040718-396 Danio rerio (Zfin) DDB0205389 Dictyostelium discoideum (Dictybase) MGI:103251 Mus musculus (Informatics) SPBC1703.07 Schizosaccharomyces pombe (Sanger)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Szeged	- P{EP}ATPCLEP2329
Harvard	- PBac{PB}ATPCL