Gene Dmel\GstD5

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General Information

Symbol

Dmel\GstD5

Species

D. melanogaster

Name

Glutathione S transferase D5

Annotation symbol

CG12242

Feature type

protein_coding_gene

FlyBase ID

FBgn0010041

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

87B8-87B8

Sequence location

3R:8,201,486..8,202,136 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Glutathione S transferase D5 is referred to in FlyBase by the symbol GstD5 (CG12242, FBgn0010041). It has the cytological map location 87B8. Its sequence location is 3R:8201486..8202136. Its molecular function is described as glutathione transferase activity. The biological processes in which it is involved are not known. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B8-87B8

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: GstD8- GstD7- GstD6- GstD5- GstD4- GstD3- GstD2- GstD1+

(Toung et al., 1993)

Overall orientation not stated: GstD10- GstD9- GstD1- GstD2+ GstD3+ GstD4+ GstD5+ GstD6+ GstD7+ GstD8+

(Sawicki et al., 2003)

Gene Model & Products

Please see the GBrowse view of Dmel\GstD5 for information on other features

Comments on Gene Model

Probable mutation in sequenced strain: premature stop codon

Probable mutation in sequenced strain: premature stop: full length CDS sequence can be obtained from FBrf0058802.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

GstD5-RA

FBtr0082572

NM_080175

651

216

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

GstD5-PA

FBpp0082044

24.7

216

7.57

NP_524914

AAF54790

Additional Polypeptide Data & Comments

Reported size (kD)

216 (aa)

(Toung et al., 1993)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VG95

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010041

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010041

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010041

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of GstD5	Class	Mutagen	Stocks	Known lesion
GstD5¹			2	Yes

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of GstD5	Class	Mutagen	Stocks	Known lesion
GstD5^GD12981		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)kar-Sz12

Useful duplication

Dp(3;3)D1a

Disrupted in

Df(3R)Exel6164

(Ryder, 2004.4.26)

Df(3R)Exel6165

(Ryder, 2004.4.26)

Df(3R)Exel7315

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12981}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 1 )

Molecular Function

CV term

Evidence

References

glutathione transferase activity

inferred from sequence or structural similarity with MGD:Gstt1; MGI:MGI:107379

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

GstD5 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

71335

Orthologs

Genome-wide drosophilid orthologs

Dsim\GD18818

Dyak\GE26178

Curated drosophilid orthologs

Dpse\GA11503

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

NEWSINFRUP00000170142
Takifugu rubripes (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v35659

w¹¹¹⁸; P{GD12981}v35659

v35660

w¹¹¹⁸; P{GD12981}v35660

Bloomington

2057

y¹ oc^R3.2; Gr22b¹ Gr22d¹ cn¹ CG33964^R4.2 bw¹ sp¹; LysC¹ lab^R4.2 MstProx¹ GstD5¹ Rh6¹ <E>

Tucson

14021-0231.36

y¹ oc^R3.2; Gr22b¹ Gr22d¹ cn¹ CG33964^R4.2 bw¹ sp¹; LysC¹ lab^R4.2 MstProx¹ GstD5¹ Rh6¹ <E>

Genomic Clones ( 2 )

cDNA Clones ( 2 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010041

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010041

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: GstD24 CG12242

(Misra, 2000.7.28)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_080175

RefSeq (Proteins)

•

NP_524914

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

Q9VG95

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

71335

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010041

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010041

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010041

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0010041

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010041

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010041

InParanoid orthologs - Eukaryotic orthologs

•

NEWSINFRUP00000170142
Takifugu rubripes (Ensembl)

Synonyms & Secondary IDs ( 10 )

Reported As

Symbol Synonym

CG12242

(Lee et al., 2003, Mount, 2001.8.14, )

DmGST24

(Ranson et al., 2001, Chelvanayagam et al., 2001, Zimniak and Benes, 2002.7.15, Toung et al., 1993)

DmGSTD5

(Sawicki et al., 2003, Chelvanayagam et al., 2001, Zimniak and Benes, 2002.7.15)

Gst

(Ranz et al., 2001, Ranz et al., 2001, )

GST

(Arking, 1998)

GstD5

GstD24

(Vodovar et al., 2005, )

gstD24

(Toung et al., 1993)

Name Synonym

Glutathione S transferase D5

(Zimniak and Benes, 2002.7.15)

Glutathione S transferase D24

Secondary FlyBase IDs

FBgn0038024

References ( 20 )

Research paper

Vodovar et al., 2005, Proc. Natl. Acad. Sci. USA 102(32): 11414--11419: Drosophila host defense after oral infection by an entomopathogenic Pseudomonas species. [FBrf0188279]
Lee et al., 2003, Curr. Biol. 13(4): 350--357: Genome-wide analyses of steroid- and radiation-triggered programmed cell death in Drosophila. [FBrf0158810]
Sawicki et al., 2003, Biochem. J. 370(2): 661--669: Cloning, expression and biochemical characterization of one Epsilon-class (GST-3) and ten Delta-class (GST-1) glutathione S-transferases from Drosophila melanogaster, and identification of additional nine members of the Epsilon class. [FBrf0158728]
Shen et al., 2003, Arch. Insect Biochem. Physiol. 53(2): 80--91: Induction of glutathione S-transferases activities in Drosophila melanogaster exposed to phenol. [FBrf0160946]
Chelvanayagam et al., 2001, Chem. Biol. Interact. 133: 256--260: Fly fishing for GSTs: a unified nomenclature for mammalian and insect glutathione transferases. [FBrf0150783]
Ranson et al., 2001, Biochem. J. 359(2): 295--304: Identification of a novel class of insect glutathione S-transferases involved in resistance to DDT in the malaria vector Anopheles gambiae. [FBrf0151633]
Ranz et al., 2001, Genome Res. 11(2): 230--239: How malleable is the eukaryotic genome? Extreme rate of chromosomal rearrangement in the genus Drosophila. [FBrf0132349]
Toung et al., 1993, J. Biol. Chem. 268(13): 9737--9746: The glutathione S-transferase D genes. A divergently organized, intronless gene family in Drosophila melanogaster. [FBrf0058802]

Supplementary material

Ranz et al., 2001, Genome Res. 11(2):: Table 1. Markers from chromosomal arm 3R of Drosophila melanogaster mapped on the Drosophila repleta chromosome 2. [FBrf0137433]

Review

Arking, 1998, Curr. Sci. 74(10): 859--864: Molecular basis of extended longevity in selected Drosophila strains. [FBrf0103502]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Zimniak and Benes, 2002.7.15, Insect GSTs.: Insect GSTs. [FBrf0150782]
Mount, 2001.8.14, Intron annotations.: Intron annotations. [FBrf0137489]
Misra, 2000.7.28, Matches of the nc2 class to CGs.: Matches of the nc2 class to CGs. [FBrf0129567]

FlyBase analysis

FlyBase Genome Annotators, 2004, Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome.: Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. [FBrf0166453]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Protein sequence record

Swiss-Prot Project Members, 2000.10.1, UniProtKB: Q9VG95: GLUTATHIONE S-TRANSFERASE D24 (EC 2.5.1.18) (DMGST24). [FBrf0131803]

Curated genome annotation

Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126651]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]