Gene Dmel\GstD2

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General Information

Symbol

Dmel\GstD2

Species

D. melanogaster

Name

Glutathione S transferase D2

Annotation symbol

CG4181

Feature type

protein_coding_gene

FlyBase ID

FBgn0010038

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

87B8-87B8

Sequence location

3R:8,197,720..8,198,367 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Glutathione S transferase D2 is referred to in FlyBase by the symbol GstD2 (CG4181, FBgn0010038). It has the cytological map location 87B8. Its sequence location is 3R:8197720..8198367. Its molecular function is described as: glutathione transferase activity; glutathione peroxidase activity. The biological processes in which it is involved are not known. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87B8-87B8

Limits computationally determined from genome sequence between P{PZ}svp⁰⁷⁸⁴² and P{lacW}Vha55^j2E9

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Overall orientation not stated: GstD8- GstD7- GstD6- GstD5- GstD4- GstD3- GstD2- GstD1+

(Toung et al., 1993)

Overall orientation not stated: GstD10- GstD9- GstD1- GstD2+ GstD3+ GstD4+ GstD5+ GstD6+ GstD7+ GstD8+

(Sawicki et al., 2003)

Gene Model & Products

Please see the GBrowse view of Dmel\GstD2 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

GstD2-RA

FBtr0082569

NM_080173

648

215

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

GstD2-PA

FBpp0082041

24.5

215

4.97

NP_524912

AAF54787

Additional Polypeptide Data & Comments

Reported size (kD)

215 (aa)

(Toung et al., 1993)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010038

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VG98

UniProtKB/TrEMBL

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

9156

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

adult stage

(Toung et al., 1993)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010038

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010038

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010038

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

GstD2^f00583

Sterility

Allele

fertile

GstD2^f00583

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of GstD2	Class	Mutagen	Stocks	Known lesion
GstD2^f00583		piggyBac transposase	2	--

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of GstD2	Mutagen	Known lesion
GstD2^D21L-UTR.hs	in vitro construct \| regulatory fusion	Yes
GstD2^D21L.hs	in vitro construct \| regulatory fusion	Yes
GstD2^GD11242	in vitro construct \| RNAi in vitro construct \| regulatory fusion	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)kar-Sz12

Useful duplication

Dp(3;3)D1a

Disrupted in

Df(3R)Exel6164

(Ryder, 2004.4.26)

Df(3R)Exel6165

(Ryder, 2004.4.26)

Df(3R)Exel7314

(Ryder, 2004.4.26)

Df(3R)Exel7315

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11242}

characterization construct

P{hs-GstD1::GstD2.D1-171-D21}

P{hs-GstD1::GstD2.D1-66-D21}

P{hs-GstD1::GstD2.D21-170-D1}

P{hs-GstD1::GstD2.D21-65-D1}

heat-shock construct

P{hs-GstD2.D21L-UTR}

P{hs-GstD2.D21L}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}GstD2^f00583

P{GT1}BG02852

P{UAST-YFP.Rab1.S25N}01

P{UAST-YFP.Rab18.S19N}03

insertion of mobile activating element

P{EP}EP564

Related Comments

Please look at the allele reports for the complete phenotype data

Dehydro-chlorinase activity of the protein is determined by biochemical analysis using gas-liquid chromatography to assess the contribution of the isozymes to the DDT-resistant phenotype.

(Tang and Tu, 1994)

Pentobarbital rapidly induces the expression of GstD2, by activating transcription.

(Tang and Tu, 1995)

Gene Ontology: Function, Process & Cellular Component ( 2 )

Molecular Function

CV term

Evidence

References

glutathione peroxidase activity

traceable author statement

(Singh et al., 2001)

glutathione transferase activity

inferred from direct assay

(Tang and Tu, 1994)

inferred from direct assay

(Sawicki et al., 2003)

inferred from sequence or structural similarity with MGD:Gstt1; MGI:MGI:107379

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 2000.10.1)

Biological Process

CV term

Evidence

References

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

GstD2 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

71332

Orthologs

Genome-wide drosophilid orthologs

Dere\GG18761

Dsim\GD18815

Dyak\GE26175

Curated drosophilid orthologs

Dpse\GA18009

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

DDB0229820
Dictyostelium discoideum (Dictybase)
DDB0231433
Dictyostelium discoideum (Dictybase)
DDB0231434
Dictyostelium discoideum (Dictybase)
SPCC191.09c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 7 )

Szeged

P{EP}EP564

Bloomington

17181

w¹¹¹⁸; P{EP}EP564

12830

w¹¹¹⁸; P{GT1}BG02852

18346

w¹¹¹⁸; PBac{WH}GstD2^f00583

9757

y¹ w^*; P{UAST-YFP.Rab1.S25N}01

23238

y¹ w^*; P{UAST-YFP.Rab18.S19N}03

Harvard

PBac{WH}GstD2^f00583

Genomic Clones ( 2 )

cDNA Clones ( 1 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

IP20147

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010038

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010038

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: GstD21 CG4181

(Whitfield, 2000.5.17)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_080173

RefSeq (Proteins)

•

NP_524912

DDBJ / EMBL / GenBank

•

UniProtKB/Swiss-Prot

•

Q9VG98

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Glutathione S-transferase, N-terminal (IPR004045)

Glutathione S-transferase, C-terminal (IPR004046)

Glutathione S-transferase, C-terminal-like (IPR010987)

Thioredoxin-like fold (IPR012336)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

71332

DEDB - Drosophila exon database: splicing graphs

•

9156

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0010038

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0010038

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0010038

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0010038

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0010038

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0010038

InParanoid orthologs - Eukaryotic orthologs

•

DDB0229820
Dictyostelium discoideum (Dictybase)
DDB0231433
Dictyostelium discoideum (Dictybase)
DDB0231434
Dictyostelium discoideum (Dictybase)
SPCC191.09c
Schizosaccharomyces pombe (Sanger)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0010038

Synonyms & Secondary IDs ( 10 )

Reported As

Symbol Synonym

CG4181

(Whitfield, 2000.5.17, )

DmGST21

(Ranson et al., 2001, Chelvanayagam et al., 2001, Zimniak and Benes, 2002.7.15, Toung et al., 1993)

DmGSTD2

(Sawicki et al., 2003, Chelvanayagam et al., 2001, Zimniak and Benes, 2002.7.15)

Gst

(Ranz et al., 2001, Ranz et al., 2001, )

GST

(Arking, 1998)

GstD2

(Willoughby et al., 2006, Zhang et al., 2007, Yepiskoposyan et al., 2006)

GstD21

(Landis et al., 2004, )

gstD21

(Akgul and Tu, 2004, Akgul and Tu, 2002, Tang and Tu, 1995, Tang and Tu, 1994, Toung et al., 1993)

Name Synonym

Glutathione S transferase D2

(Zimniak and Benes, 2002.7.15, Zhao et al., 2008)

Glutathione S transferase D21

Secondary FlyBase IDs

FBgn0038021

References ( 27 )

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All references relating to this gene ( 27 )

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Recent research papers ( 2 )

Willoughby et al., 2006, Insect Biochem. Molec. Biol. 36(12): 934--942: A comparison of Drosophila melanogaster detoxification gene induction responses for six insecticides, caffeine and phenobarbital. [FBrf0193014]
Yepiskoposyan et al., 2006, Nucleic Acids Res. 34(17): 4866--4877: Transcriptome response to heavy metal stress in Drosophila reveals a new zinc transporter that confers resistance to zinc. [FBrf0193346]

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Recent reviews (0)

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