A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\pwn

General Information
SymbolDmel\pwnSpeciesD. melanogaster
NamepawnAnnotation symbolCG11101
Feature typeprotein_coding_geneFlyBase IDFBgn0003174
Created / Updated2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)2RRecombination map2-55.4
Cytogenetic map43A3-43A3Sequence location2R:3,182,813..3,187,907 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene pawn is referred to in FlyBase by the symbol pwn (CG11101, FBgn0003174). It has the cytological map location 43A3. Its sequence location is 2R:3182813..3187907. Its molecular function is described as calcium ion binding. It is involved in the biological processes: bristle morphogenesis; phototransduction. 13 alleles are reported. The phenotypes of these alleles are annotated with: macrochaeta; eye; cornea; chaeta; retina. It has one annotated transcript and one annotated polypeptide.

hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
pwn: pawn
Homozygous semilethal as zygote (survival 2%); escapers have dark brown eyes and show tanning of cornea cuticle (Garcia-Bellido and Dapena, 1973). Cell viable in homozygous clones, which have truncated bristles with pale tips and pin-shaped trichomes with basal spurs and thin, transparent hairs (Garcia-Bellido, 1973, 1974; Lawrence and Morata, 1976). Useful as cell marker.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
43A3-43A3  
Limits computationally determined from genome sequence between P{lacW}vimark16722 and P{lacW}cosk16101  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
2-55.4
(Ashburner et al., 1981)
Left of (cM)
(Ashburner et al., 1981)
Right of (cM)
(Ashburner et al., 1981, Grau and Simpson, 1987)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\pwn for information on other features
detailed view FBtr0088962 FBpp0088036 FBti0042393 FBti0053039
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
pwn-RA
FBtr0088962
 NM_136423
  3924
  1307
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
pwn-PA  
FBpp0088036  
139.9  
1307  
6.19  
  NP_610267
  AAF59274
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Aspartic acid and asparagine hydroxylation site (IPR000152)
EGF-like, type 3 (IPR000742)
EGF-like calcium-binding (IPR001881)
EGF-like region, conserved site (IPR013032)
EGF calcium-binding (IPR013091)
EGF, extracellular (IPR013111)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AE003841
AAF59274
 
CO178486
 
CO287290
 
CO318230
 
CO339524
 
EC061950
 
EC081158
 
EC089685
 
EC195763
 
EC206802
 
EC252600
 
EC253003
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        viable | germ-line clone | recessive
        lethal
        lethal (with Df(2R)CA58)
        lethal (with Df(2R)pwn5)
        lethal (with Df(2R)sple-D1)
        lethal | embryonic stage
        semi-lethal | recessive
        Other Phenotypes
        Allele
        visible
        visible (with Df(2R)pwn5)
        visible | recessive
        neurophysiology defective
        Sterility
        Allele
        fertile
        Phenotype manifest in
        Allele
        macrochaeta
        eye
        cornea
        chaeta
        microvillus & rhabdomere | distal
        retina
        hide Classical Alleles ( 11 )
        For All Classical Alleles Show

        Allele of pwnClassMutagenStocksKnown lesion
        pwn12A0 Yes
        pwn14S7
          		0 Yes
          pwn126 --
          pwn20 Yes
          pwn30 --
          pwn41 Yes
          pwn50 --
          pwn60 --
          pwn70 --
          pwnf048312 --
          pwnunspecified
            		0 --
            hide Alleles Carried on Transgenic Constructs ( 2 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of pwnClassMutagenStocksKnown lesion
            pwnGD1490 Yes
            pwnGD26451 Yes
            hide Aneuploid Aberrations
            Useful deficiency
             
             
            Disrupted in
            (Arruda and Dolph, 2003)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Roote and Ashburner, 2005.6)
            (Roote and Ashburner, 2005.6)
            (Roote and Ashburner, 2005.6)
            (Heitzler et al., 1993)
            (Ashburner et al., 1981)
            (Ashburner et al., 1981)
            (Arruda and Dolph, 2003, Heitzler et al., 1993)
            (Gubb and Garcia-Bellido, 1982)
            (Arruda and Dolph, 2003, Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            Not disrupted in
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Roote and Ashburner, 2005.6)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            (Heitzler et al., 1993)
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            UAS construct
            P{GD149}
            NA
            P{GD2645}
            NA
            Insertions
            Type of insertions
            Name
            Expression data
            miscellaneous insertions
            PBac{WH}pwnf04831
            NA
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            hide Gene Ontology: Function, Process & Cellular Component ( 3 )
            hide Molecular Function
            CV term
            Evidence
            References
            calcium ion binding
            inferred from electronic annotation with InterPro:IPR001881
            (FlyBase Curators et al., 2004-)
            hide Biological Process
            CV term
            Evidence
            References
            bristle morphogenesis
            inferred from mutant phenotype
            (Heitzler et al., 1993)
            phototransduction
            inferred from mutant phenotype
            (Arruda and Dolph, 2001)
            hide Cellular Component
            CV term
            Evidence
            References
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            pwn allele
            Gene
            References
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
             
            Linkouts
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Functional Complementation between Species
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 30 )
            Harvard
            -
            PBac{WH}pwnf04831
            VDRC
            v5434
            w1118; P{GD2645}v5434
            Bloomington
            18807
            w1118; PBac{WH}pwnf04831
            8877
            Dp(?;2)bwD, pwn4 bwD/CyO
            5742
            Dp(3;3)Cam30T, pr1 pwn1 cn1/CyO
            6327
            T(1;2)scS2, pwn1/In(2LR)Gla, wgGla-1
            Kyoto
            102053
            T(1;2)scS2, pwn1 / In(2LR)Gla
            108914
            Df(2R)Px1, al1 dpov1 pwn1/SM5
            108398
            Dp(3;3)Cam30T, pr1 pwn1 cn1/CyO
            106224
            pwn1 en1/SM5; mwh1 jv1/TM1
            102054
            Df(1)sc19 / FM6 ; pwn1 cn1 / In(2L)Cy
            101815
            f36a ; pr1 pwn1 P{f+13}44C P{f+13}47B / CyO
            hide Genomic Clones ( 1 )
            hide cDNA Clones ( 4 )
            Please Note
            This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            hide Antibody Information
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for database merge of
            Source for merge of: pwn CG11101
            (Arruda and Dolph, 2001)
            Additional comments
            "l(2)43Bb" may correspond to "CG11101".
            (Schnorr et al., 2001)
            hide Comments About Role
            hide Comments About Molecular Function
            hide Other Comments
            Mutant alleles are useful as markers in clonal analysis.
            (Lawrence et al., 1986)
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            RefSeq (Transcripts)
            •
            RefSeq (Proteins)
            •
            DDBJ / EMBL / GenBank
            •
            UniProtKB/TrEMBL
            •
            Other Crossreferences
            InterPro domains - A database of protein families, domains, and functional sites
            •
            Aspartic acid and asparagine hydroxylation site (IPR000152)
            EGF-like, type 3 (IPR000742)
            EGF-like calcium-binding (IPR001881)
            EGF-like region, conserved site (IPR013032)
            EGF calcium-binding (IPR013091)
            EGF, extracellular (IPR013111)
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            DEDB - Drosophila exon database: splicing graphs
            •
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            •
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            •
            FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
            •
            GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            InParanoid orthologs - Eukaryotic orthologs
            •
            PANTHER - Protein classification by function, families, and pathways
            •
            hide Synonyms & Secondary IDs ( 6 )
            Reported As
            Symbol Synonym
            CG11101
            (Schnorr et al., 2001, )
            CT31067
            (Hynes and Zhao, 2000)
            l(2)pwn
            (Heitzler et al., 1993)
            pwn
            (Lawrence et al., 2004)
            Name Synonym
            N-like
            (Hynes and Zhao, 2000)
            pawn
             
            Secondary FlyBase IDs
            • FBgn0033157
            hide References ( 35 )
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