Gene Dmel\eIF-4a

General Information
Symbol	Dmel\eIF-4a	Species	D. melanogaster
Name	Eukaryotic initiation factor 4a	Annotation symbol	CG9075
Feature type	protein_coding_gene	FlyBase ID	FBgn0001942
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	26B2-26B2	Sequence location	2L:5,981,759..5,985,572 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Eukaryotic initiation factor 4a is referred to in FlyBase by the symbol eIF-4a (CG9075, FBgn0001942). It has the cytological map location 26B2. Its sequence location is 2L:5981759..5985572. Its molecular function is described as: RNA helicase activity; translation initiation factor activity; RNA cap binding; mRNA binding; single-stranded DNA-dependent ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATP binding. It is involved in the biological processes: dorsal/ventral axis specification; translational initiation; imaginal disc growth; instar larval development; DNA unwinding during replication; regulation of alternative nuclear mRNA splicing, via spliceosome; mitotic spindle elongation; mitotic spindle organization and biogenesis. 58 alleles are reported. The phenotypes of these alleles are annotated with 23 unique terms, many of which group under: blastoderm embryo; extended germ band embryo; gastrula embryo; organ system; embryonic tagma; nervous system; cuticle; integumentary system; dorsal thoracic disc; parasegment 14; parasegment 2; cephalopharyngeal skeleton; peripheral nervous system; late extended germ band embryo. It has 4 annotated transcripts and 4 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 26B2-26B2 Limits computationally determined from genome sequence between P{EP}chic^EP713&P{EP}chic^EP1095 and P{lacW}Gef26^k13720
Experimentally Determined Cytological Location
Cytogenetic map Notes References 25D5-26D1 (Li and Gelbart, 2002) 26B1-26B2 (determined by in situ hybridisation) (Spradling et al., 1999) 26A5-26A7 26A5--8 26A7--8 26A7--9 26A8--9 26B1--2 26B8--9 (BDGP Project Members, 1994-1999) 26A7-26A9 (determined by in situ hybridisation) 27A7--27A9 (determined by in situ hybridisation) (Dorn et al., 1993)
Experimentally Determined Recombination Data
Location
Left of (cM)	P{EP}CG13993^EP570 P{EP}EP2273 P{lacW}l(2)k09923^k09923 P{lacW}l(2)k09022^k09022 P{lacW}hoip^k07104 b P{lacW}l(2)k05404^k05404 (Li et al., 2005)
Right of (cM)	P{lacW}vri^k05901 P{EP}Gpdh^EP2304 P{lacW}l(2)k06502^k06502 P{lacW}chic^k13321 dp (Li et al., 2005)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: ifc+ eIF-4a- chic+ (Basu and Li, 1998)
Gene Model & Products
Please see the GBrowse view of Dmel\eIF-4a for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) eIF-4a-RA FBtr0079175 NM_164668 1689 403 eIF-4a-RB FBtr0079177 NM_164669 1910 403 eIF-4a-RC FBtr0079176 NM_057247 1571 403 eIF-4a-RD FBtr0079178 NM_164670 1792 403
Additional Transcript Data & Comments
Reported size (kB)	2.2, 2.0 (northern blot) (Cooley et al., 1992) 1.9, 1.75 (northern blot) (Dorn et al., 1993)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein eIF-4a-PA FBpp0078806 45.9 403 5.34 NP_723137 AAF52317 eIF-4a-PB FBpp0078808 45.9 403 5.34 NP_723138 AAN10567 eIF-4a-PC FBpp0078807 45.9 403 5.34 NP_476595 AAN10566 eIF-4a-PD FBpp0078809 45.9 403 5.34 NP_723139 AAN10568
Additional Polypeptide Data & Comments
Reported size (kD)	402 (aa) (Dorn et al., 1993)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0001942
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA helicase, ATP-dependent, DEAD-box, conserved site (IPR000629) DNA/RNA helicase, C-terminal (IPR001650) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) DEAD-like helicase, N-terminal (IPR014001) RNA helicase, DEAD-box type, Q motif (IPR014014) Helicase, superfamily 1 and 2, ATP-binding (IPR014021)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201849 AA201904 AA202043 AA202058 AA202343 AA202523 AA202707 AA202885 AA246225 AA246437 AA246506 AA247040 AA263702 AA263703 AA263740 AA264124 AA264232 AA264404 AA264540 AA390312 AA390330 AA390395 AA390651 AA390892 AA390978 AA391457 AA391976 AA392388 AA392501 AA438988 AA439755 AA439819 AA440183 AA440932 AA441300 AA538657 AA539088 AA539371 AA539422 AA540050 AA540710 AA540831 AA567273 AA567376 AA567552 AA694788 AA694826 AA695516 AA695987 AA696353 AA696408 AA696520 AA697086 AA697222 AA697323 AA697431 AA697788 AA697802 AA697804 AA697829 AA698722 AA698991 AA735106 AA735346 AA735456 AA735836 AA735931 AA736007 AA801530 AA801735 AA801806 AA801846 AA801924 AA801960 AA802051 AA802692 AA802790 AA802848 AA803048 AA803391 AA803571 AA803621 AA803725 AA803938 AA804095 AA816632 AA816793 AA817113 AA817502 AA817518 AA817542 AA817603 AA820066 AA820093 AA820379 AA820516 AA820578 AA820735 AA820837 AA820989 AA821083 AA821157 AA940905 AA940910 AA941322 AA941530 AA941537 AA941629 AA941652 AA941803 AA941848 AA942119 AA942175 AA942326 AA942422 AA942582 AA948954 AA949207 AA949330 AA949362 AA949550 AA950096 AA950102 AA950248 AA950323 AA950469 AA950703 AA950705 AA950774 AA950787 AA950815 AA950975 AA951108 AA951316 AA951420 AA951579 AA951658 AA952057 AA952062 AA952073 AA952180 AA952215 AA978383 AA979078 AA979291 AA979421 AA979461 AA979576 AA979694 AA990658 AA990687 AA990720 AC004268 AC004758 AC092216 AE003612 AAF52317 AE003612 AAN10566 AE003612 AAN10567 AE003612 AAN10568 AF145621 AI061704 AI061711 AI061915 AI062172 AI062369 AI063589 AI063841 AI063866 AI064029 AI064134 AI064322 AI064349 AI064634 AI107476 AI107807 AI107939 AI107944 AI109863 AI110001 AI110016 AI110100 AI113781 AI134236 AI135016 AI135037 AI135038 AI135217 AI135282 AI135503 AI135863 AI135955 AI238013 AI238055 AI238740