HomeGene Dmel\Pka-C1
| General Information | ||||
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| Symbol | Dmel\Pka-C1 | Species | D. melanogaster | |
| Name | cAMP-dependent protein kinase 1 | Annotation symbol | CG4379 | |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0000273 | |
| Created / Updated | 2003-12-01/2003-12-01 | |||
| Genomic Location | ||||
| Chromosome (arm) | 2L | Recombination map | ||
| Cytogenetic map | 30C5-30C5 | Sequence location | 2L:9,684,656..9,699,293 [-] | |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | The gene cAMP-dependent protein kinase 1 is referred to in FlyBase by the symbol Pka-C1 (CG4379, FBgn0000273). It has the cytological map location 30C5. Its sequence location is 2L:9684656..9699293. Its molecular function is described as: cAMP-dependent protein kinase activity; protein binding; protein serine/threonine kinase activity; ATP binding. It is involved in the biological processes described with 21 unique terms, many of which group under: anatomical structure development; anterior/posterior axis specification; learning and/or memory; oocyte axis determination; learning; response to chemical stimulus; organ morphogenesis; regulation of biological process; oocyte anterior/posterior axis determination; second-messenger-mediated signaling; olfactory learning; establishment and/or maintenance of cytoskeleton polarity; biopolymer modification; regulation of molecular function; locomotory behavior; sleep; memory. 83 alleles are reported. The phenotypes of these alleles are annotated with 41 unique terms, many of which group under: adult segment; organ system; adult; peripheral nervous system; nervous system; adult mesothoracic segment; thoracic segment; imaginal precursor; follicle cell; lateral plate. It has 3 annotated transcripts and 3 annotated polypeptides. | |||
| External Summaries | ||||
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Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
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| Gene/Allele symbols may differ from current usage | Cos: Costal (P. Simpson)
Mirror-image duplications of wings and halteres;
duplications of wing begin in mid-costal region and may
include entire anterior compartment; posterior compartments
unaffected, even when transformed into anterior compartments
in en homozygotes. Penetrance of Cos higher when maternally
than when paternally inherited. Class I alleles are homozygous viable and display pattern duplications in both heterozygotes and homozygotes. Penetrance and expressivity variable
and may overlap wild type. Class II alleles are lethal when
homozygous or in heteroallelic combination with one another;
they sometimes display pattern duplications in heterozygotes.
Class III alleles are homozygous lethal and have no dominant
phenotype. Flies heterozygous for alleles of any of the three
classes display the phenotype when simultaneously heterozygous
for lethal alleles or deletions of cos; the heterozygous
expression of Cos is dependent on the number of cos+ alleles
present, with the severity of wing duplication decreasing as
the number of cos+ alleles increases from one to three. Flies
heterozygous for Cos and homozygous or hemizygous for some
viable alleles of cos die as pharate adults and display pattern duplications in the anterior compartment of every body
segment. Homozygotes of class II and IIIK alleles die as
embryos with abnormal cuticular patterns; failure to develop
of variable extents of the anterior end of the embryo, i.e.
head or head and thorax, as well as mirror image duplications
of anterior denticle belts of abdominal segments, or more frequently simply disturbed denticle polarity. Embryos that are
simultaneously homozygous for cosV alleles and Cos3 are more
severely affected, with some exhibiting a bicaudal phenotype.
cosV1 Cos2/Df(2R)CA58 flies survive poorly, but show pattern
duplications in the anterior compartments of all segments.
Revertants of Cos are viable and wild type in phenotype; as
they are presumably null alleles, Cos mutations are presumed
to be gain of function alleles.
Pka-C1
Encodes one of the isoforms of the catalytic subunit
form of Pka.
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | ||||
Cytogenetic map Evidence for location 30C5-30C5
Limits computationally determined from genome sequence between P{EP}EP361&P{EP}peloEP2160 and P{lacW}zf30Ck02506&P{EP}undEP424
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| Experimentally Determined Cytological Location | ||||
Cytogenetic map Notes References 30C1-30C2 (determined by in situ hybridisation)
30C1-30C2 (determined by in situ hybridisation)
30C-30C (determined by in situ hybridisation)
30C1-30C2 30C1--9
30C-30C (determined by in situ hybridisation)
30C1-30C6 (determined by in situ hybridisation) 30C (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | ||||
| Location | ||||
| Left of (cM) | ||||
| Right of (cM) | ||||
| Notes | ||||
| Molecular Map Data | ||||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References | ||||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\Pka-C1
for information on other features
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| Comments on Gene Model | ||||
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Transcript Data
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| Annotated Transcripts | ||||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | ||||
| Additional Transcript Data & Comments | ||||
| Reported size (kB) | 5.1, 4.1, 3.4, 2.4 (northern blot) 5.1, 4.1, 3.4 (northern blot) | |||
| Comments | ||||
| External Data | ||||
| Crossreferences | ||||
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Polypeptide Data
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| Annotated Polypeptides | ||||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | ||||
| Reported size (kD) | 40 (kD observed) 352 (aa) 353 (aa) | |||
| Comments | ||||
| External Data | ||||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Protein kinase, core (IPR000719)
Protein kinase, C-terminal (IPR000961)
Serine/threonine protein kinase (IPR002290)
Serine/threonine protein kinase, active site (IPR008271)
Protein kinase-like (IPR011009)
Protein kinase ATP binding, conserved site (IPR017441)
Serine/threonine protein kinase-related (IPR017442)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | |||
| UniProtKB/Swiss-Prot | ||||
| UniProtKB/TrEMBL | ||||
| Maps to | ||||
| Does NOT map to | ||||
| Identified with | ||||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References point mutation Pka-C1[G9] 2L:9,697,467..9,697,467 reported_pr_change=R34@ evidence=experimental na_change=C9697467T pr_change=R34|Pka-C1-PA,R34|Pka-C1-PB,R34@|Pka-C1-PC reported_na_change=C2223T point mutation Pka-C1[B12] 2L:9,697,185..9,697,185 evidence=experimental na_change=G9697185A pr_change=G128S|Pka-C1-PA,G128S|Pka-C1-PB,G128S|Pka-C1-P C reported_na_change=G2505A reported_pr_change=G128S point mutation Pka-C1[C2] 2L:9,697,184..9,697,184 evidence=experimental na_change=G9697184A pr_change=G128D|Pka-C1-PA,G128D|Pka-C1-PB,G128D|Pka-C1-P C reported_na_change=G2506A reported_pr_change=G128D point mutation Pka-C1[Cos-2] 2L:9,697,179..9,697,179 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G9697179A pr_change=E130K|Pka-C1-PA,E130K|Pka-C1-PB,E130K|Pka-C1-P C reported_pr_change=E130K point mutation Pka-C1[Cos-9] 2L:9,697,179..9,697,179 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G9697179A pr_change=E130K|Pka-C1-PA,E130K|Pka-C1-PB,E130K|Pka-C1-P C reported_pr_change=E130K point mutation Pka-C1[Cos-3] 2L:9,697,050..9,697,050 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G9697050A pr_change=E173K|Pka-C1-PA,E173K|Pka-C1-PB,E173K|Pka-C1-P C reported_pr_change=E173K point mutation Pka-C1[A13] 2L:9,697,010..9,697,010 evidence=experimental na_change=C9697010T reported_pr_change=T186M pr_change=T186M|Pka-C1-PA,T186M|Pka-C1-PB,T186M|Pka-C1-P C reported_na_change=C2680T point mutation Pka-C1[Cos-8] 2L:9,697,001..9,697,001 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=G9697001A pr_change=G189D|Pka-C1-PA,G189D|Pka-C1-PB,G189D|Pka-C1-P C reported_pr_change=G189D point mutation Pka-C1[H3] 2L:9,696,969..9,696,969 na_change=A9696969G evidence=experimental pr_change=T200A|Pka-C1-PA,T200A|Pka-C1-PB,T200A|Pka-C1-P C reported_na_change=A2721G reported_pr_change=T200A point mutation Pka-C1[C4] 2L:9,696,962..9,696,962 evidence=experimental na_change=G9696962A pr_change=C202Y|Pka-C1-PA,C202Y|Pka-C1-PB,C202Y|Pka-C1-P C reported_na_change=G2728A reported_pr_change=C202Y point mutation Pka-C1[H2] 2L:9,696,959..9,696,959 evidence=experimental na_change=G9696959A pr_change=G203D|Pka-C1-PA,G203D|Pka-C1-PB,G203D|Pka-C1-P C reported_na_change=G2731A reported_pr_change=G203D point mutation Pka-C1[B10] 2L:9,696,911..9,696,911 evidence=experimental na_change=A9696911T pr_change=N219I|Pka-C1-PA,N219I|Pka-C1-PB,N219I|Pka-C1-P C reported_na_change=A2779T reported_pr_change=N219I point mutation Pka-C1[B3] 2L:9,696,671..9,696,671 evidence=experimental na_change=G9696671A pr_change=W299|Pka-C1-PA,W299|Pka-C1-PB,W299@|Pka-C1-P C reported_na_change=G3019A reported_pr_change=W299@ point mutation Pka-C1[D30] 2L:9,696,529..9,696,529 evidence=experimental na_change=T9696529A reported_na_change=T3161A reported_pr_change=C346@ pr_change=C346|Pka-C1-PA,C346|Pka-C1-PB,C346@|Pka-C1-P C transposable element target site duplication P{lacW}Pka-C1[k08918] 2L:9,698,578..9,698,585 comment=insertion associated host repeat evidence=experimental | ||||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
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| Crossreferences | ||||
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Expression Data
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| FlyBase-Curated Data | ||||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | |||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
adult stage
optic lobe
adult stage
mushroom body
oogenesis stage,adult stage
germarium
adult stage
oogenesis stage,adult stage
oocyte
adult stage
adult brain
adult stage
thoracic ganglion
embryonic stage-adult stage
oogenesis stage,adult stage
nurse cell | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
adult stage | female
ovary
adult stage
β-lobe
larval stage | third instar
larval brain
adult stage
α-lobe
embryonic stage-adult stage
adult stage
mushroom body
adult stage
γ-lobe
adult stage
calyx of mushroom body
adult stage | male
testis
larval stage | third instar
dorsal mesothoracic disc
adult stage | male
male accessory gland | ||||
| Marker for | ||||
| Subcellular Localization | ||||
| CV Term | ||||
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele
Other Phenotypes Allele Sterility Allele Phenotype manifest in Allele | ||||