Clone Dmel\RH13874

General Information
Symbol	Dmel\RH13874	Species	D. melanogaster
Name	RH13874	FlyBase ID	FBcl0267567
Feature type	cDNA_clone	Created / Updated	2005-11-02/2006-08-19
Computed gene(s)

Collection Status	N/A
Known Problems	No poly A tail
Library	RH
Strain	y; cn bw sp
Tissue Source	adult stage head
Vector	pFLC-I
Sequence Data of the Insert
5' Sequence
Total bases
GenBank
5' sequence data
5' Sequence
Total bases	648
GenBank	BI609130
5' sequence data	CCAAATTATGTCGATTCTGCGCTGCATCAGCACTATCTCCGATTGCAGCA GCGAATTGAGCGGCTTTTAGCTTTGGATTTGGCATATGGCCTGATGTCCA ATTTCTGATGGCTTTACTTTTGCTTTTGCCTTTGCTTTTGGGCTGACTGA CTAAGCCTAAGCTTTTGTTTTGGCAATCAAAAGGAGCTTATTTCATGGAA CTGCACATGCCACACACATTACACTGCTCGAAATGCCAGATTAAGCGGGA AAACTATATACATAGTTGGAATGTAATGAAACATCATCGCCAACTTTGGG TACACAGTGCTTATTAATGGGTAATTTCCGATAAGTAATGCATTTAATCT TAATTTATTTTTATAAACAATACTTGTTTTTTTTTTTTTAACTACTGAAA ATACTTTAATAATTTGTCAGTTAGTTTTTGAATAAAATACAGTTGCAAAT AATATTTACCACAGCCATCGTTTTATTAATGAATAGGTAATGAACAGGTA AGTGGAATTTTCTTCCAGTGTAAGCACTTGATCCGACTCTGATTGCAACA CCAATCGGCTGTCCCAGCTGTGCCACCACTCAGCTCCACAGCTGCACTGA CGACGATCCAATCCGATGGGTGCGAAGATCATCTGCGTGGCTCGTGGA
3' Sequence
Total bases
GenBank
3' sequence data
3' Sequence
Total bases	650
GenBank	CK134988
3' sequence data	ACCATGTGCATAATCTCGGCGGGCAACTCTCCGCCAGGCTGAGATTGCGC CCATCGCGCATATAGGCGAAGCTCGGGGGATGCAGGCTGGCCATTTCGAA TGGCTGGTACTGGTGGCGCTTCGGTGGTGGCTTCGTCTAAACTTGGATGG CTGGCTACTCTGCTGCGGCGACTACTCGCTGTCCATAGCAGGACGGCTGA CTCTCGGGACGCGAACTCGGCGGCAATGCCTGGGGCGGACCATGGTCGTC TGGTCTTTATATGGCCACTTGTGTACAACGCGTCTTAGACTAATTGCCCT AATCCAATTAGCCGGCACTTGGCCAATCCAAAGCTAACCGTTCGAATTGG GCTTTCGATTGCCGCTCGTTTGCTGCGTTGGATTAGCAGCAGTCGCGTCA TTTTGAAATCGTCTTAGCCGCCACAACGGACAAGGTCAAGTTTGAGGTAC CACCCACCCGGCAATTAGATTAAGACAAACGCTTTCCTGCTTCCTCAAGT TCACGTTTTACTAACACACTCTGGACTCGACTCTCTCTCTCAGGAGTGCC AATGGTAGCCCAGCTTGCGGGCGCCCCTCATCACGGAGGACAGCTGAAAC TGTAGGCTGCCGTACGACTCCATAGAGAGTCGCTTGCACTTCAGGTTGTC
Library Information
Comments	The RH (Riken head) library was made from RNA extracted from Drosophila adult heads, from the isogenic y; cn bw sp strain, polyA+ selected once, RNA made by Ling Hong. cDNA was synthesized by priming with the oligo(dT) primed adapter (5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3'). The first strand was synthesized in presence of trehalose, which increases the full-length cDNA synthesis (Carninci, P. et. al., PNAS 95: 520-524; Carninci, P. et al., Methods Enzymol. 303: 19-44). Subsequently, full-length cDNA was selected with the biotinylated cap-trapper (Carninci, P. et al., Genomics 37: 327-336). A linker was then ligated to the single-strand cDNA following the published protocol (Shibata, Y., et al., Biotechniques, in press). Subsequently, the cDNA was normalized by using RoT=1.0 as published (Carninci, P., et al., Genome Res. 10: 1617-1630). Second strand cDNA was primed with the (5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3') primer. After restriction digestion of the hemimethylated cDNA with BamHI and XhoI, thecDNA was cloned in the lambda FLC-I vector. Subsequently, the library was bulk-excised into pFLC-I plasmid as described (Carninci, P., et al., Genomics, 2001, 77:79-90). cDNAs were transformed into DH5-alpha TonA strain.
Library Synonyms
Reported As
Symbol Synonym
Library References
Research paper	Stapleton et al., 2002, Genome Res. 12(8): 1294--1300 The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 0 )
Research paper	Stapleton et al., 2002, Genome Res. 12(8): 1294--1300 The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]