Clone Dmel\LP21351

General Information
Symbol	Dmel\LP21351	Species	D. melanogaster
Name	LP21351	FlyBase ID	FBcl0182621
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\lark

Collection Status	BDGP
Known Problems	none
Library	LP
Strain	y; cn bw sp
Tissue Source	larval stage whole organism & pupal stage whole organism
Vector	pOT2
Sequence Data of the Insert
5' Sequence
Total bases	595
GenBank	CK661696
5' sequence data	GACGAAGTGCGAATCGATTTGGAAAAATCTGCTGCTCGGCCTGGAAAGTA GAAAGTATAAAGCTCTGCGTTTATAATTTAATTTGCAGCCAAAGCCCGCT GCCGATCAGGTATCGCGTTTGTTCGTCGCCGTTGTCGTCCTCCAACCGCG GTATATACATACAAACATATAGGCAAATATATATAAATATATTTTCGACG CAGCCGCCGCCGCCGCCGCCGCGTAGATATCCATTTTCCGGGATTACCCC ACCCAGAGTGATATAAACGATATAACCGTGGACGACCACGCAGCGGAACC CGCTGCCGCCATGCCCGGAGCCGGCACGTTCAAGTTATTCATCGGGAATC TTGACGAGAAGACACAGGCCACCGAGCTGCGTGCACTGTTCGAGAAGTAC GGTACCGTCGTCGAGTGCGACGTGGTGAAGAACTATGGCTTCGTTCACAT GGAGACGGAGCAGCAGGGTCGCGATGCCATACAGAATCTGAACGGTTATA CGCTGAACGAGTTCGCCATCAAGGTGGAGGCGGCCAAGAGCCGGCGGGCG CCCAATACGCCGACCACGAAAATCTTCGTGGGAAATCTAACGGAC
Library Information
Comments	mRNA source--varying stages of larvae and early pupae, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
Library Synonyms
Reported As
Symbol Synonym	LP (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0182621
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]