Clone Dmel\LD12790

General Information
Symbol	Dmel\LD12790	Species	D. melanogaster
Name	LD12790	FlyBase ID	FBcl0153946
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\lark

Collection Status	BDGP
Known Problems	none
Library	LD_pBS
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pBluescript_SK(-)
Sequence Data of the Insert
5' Sequence
Total bases	536
GenBank	AA440971
5' sequence data	TCACACGGTCATATTGCGGACGAATGCGAATCGATTTGGAAAAATCTGCT GCTCGGCCTGGAAAGTAGAAAGTATAAAGCTCTGCGTTTATAATTTAATT TGCAGCCAAAGCCCGCTGCCGATCAGCCGCCGCCGCCGCCGCCGCGTAGA TATCCATTTTCCGGGATTACCCCACCCAGAGTGATATAAACGATATAACC GTGGACGACCACGCAGCGGAACCCGTCGCGCCATGCCCGGAGCCGGCACG TTCAAGTTATTCATCGGGAATCTTGACGAGAAGACACAGGCCACCGAGCT GCGTGCACTGTTCGAGAAGTACGGTACCGTCGTCGAGTGCGACGTGGTGA AGAACTATGGCTTCGTTCACATGGAGACGGAGCAGCAGGGTCGCGATGCC ATACAGAATCTGAACGGTTATACGCTGAACGAGTTCGCCATCAAGGTGGA GGCGGCCAAGAGCGGCGGGCGCCTCAATACGCCGACCACGAAAATCTTCG TGGGAAATCTAACGGACAAGACGCGGGCGCCGGAGT
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pBS (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0153946
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]