Clone Dmel\GH27111

General Information
Symbol	Dmel\GH27111	Species	D. melanogaster
Name	GH27111	FlyBase ID	FBcl0111109
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\lark

Collection Status	BDGP
Known Problems	none
Library	GH
Strain	y; cn bw sp
Tissue Source	adult stage head
Vector	pOT2
Sequence Data of the Insert
5' Sequence
Total bases	635
GenBank	AI514057
5' sequence data	ATTTGGAAAAATCTGCTGCTCGGCCTGGAAAGTAGAAAGTATAAAGCTCT GCGTTTATAATTTAATTTGCAGCCAAAGCCCGCTGCCGATCAGCCGCCGC CGCCGCCGCCGCGTAGATATCCATTTTCCGGGATTACCCCACCCAGAGTG ATATAAACGATATAACCGTGGACGACCACGCAGCGGAACCCGCTGCCGCC ATGCCCGGAGCCGGCACGTTCAAGTTATTCATCGGGAATCTTGACGAGAA GACACAGGCCACCGAGCTGCGTGCACTGTTCGAGAAGTACGGTACCGTCG TCGAGTGCGACGTGGTGAAGAACTATGGCTTCGTTCACATGGAGACGGAG CAGCAGGGTCGCGATGCCATACAGAATCTGAACGGTTATACGCTGAACGA GTTCGCCATCAAGGTGGAGGCGGCCAAGAGCCGGCGGGCGCCCAATACGC CGACCACGAAAATCTTCGTGGGAAATCTAACGGACAAGACGCGGGCGCCG GAGGTGCGGGAACTGTTTCAGAAATACGGCACCGTCGTCGAATGCGACAT TGTGCGCAACTATGGTTTTGTCCATCTGGACTGTGTCGGTGATGTGCANG ACGCCATTAAGGAGCTGAACGGTCGCGTTGTCGAT
Library Information
Comments	The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, we chose to remake the head library. mRNA source--adult heads, from an isogenic y; cn bw sp strain, polyA+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
Library Synonyms
Reported As
Symbol Synonym	GH (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]